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Shandong Youyunpu photoelectric Technology Co., LTD

Youyunpu Optoelectronic Tech

Name：Jenny Li

Tel：+86 19963093538

Email：jennyli0927@163.com

Address:Oulong Science and TechnologyPark,Jinma Road,Weifang City,Shandong Province,China

Current location：HOME>PRODUCT>Program-controlled Sealer>Program-controlled Quantitative Sealing Machine
Program-controlled Quantitative Sealing Machine

Program-controlled Quantitative Sealing MachineYP-FKJ


【Introduction】:Youyunpu Optoelectronic Tech specializes in R&D & production of Program-controlled Quantitative Sealing Machine, with custom services. Complete Program-controlled Quantitative Sealing Machine models, high detection accuracy, trusted by customers.

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Product Details

I. Product Overview

The program-controlled quantitative sealing machine is an electrically driven device with intelligent circuits and a dedicated internal heating roller, specially designed for modern packaging and production. It is used for sealing 51-well and 97-well quantitative trays, and works with microbial detection reagents to provide a simple, fast and accurate experimental scheme for quantitative detection of total coliforms, fecal (thermotolerant) coliforms and Escherichia coli in water samples.
The 51-well and 97-well quantitative trays are semi-automatic quantitative methods designed based on the traditional Most Probable Number (MPN) statistical model, which can provide quantitative results. This method can replace and outperform traditional methods (membrane filtration and multiple-tube fermentation) for water microbial detection.

II. Scope of Application

For rapid detection, field portable use, emergency monitoring and quantitative detection of total coliforms, fecal (thermotolerant) coliforms and Escherichia coli in water samples.

III. Compliance Standards

  • GB 3838-2002 Environmental Quality Standards for Surface Water

  • CJ/T 206-2005 Water Quality Standards for Urban Water Supply

  • GB/T 5750.12-2023 Standard Examination Methods for Drinking Water

  • CJ/T 51-2018 Standard Examination Methods for Urban Wastewater – Determination of Thermotolerant Coliforms by Enzyme Substrate Method

  • HJ 1001-2018 National Environmental Protection Standard – Water Quality – Determination of Total Coliforms, Fecal Coliforms and Escherichia coli

IV. Product Description

The enzyme substrate detection system consists of four components: program-controlled quantitative sealing machine, 51/97-well quantitative detection tray, 100 mL quantitative bottle, and enzyme substrate detection reagent.
Applicable water samples: drinking water, source water, bottled water, reclaimed water, secondary water supply, pipe network water, wastewater, food processing water, livestock water, medical water, etc.
Advantages & Features
The enzyme substrate method is a standard method for Escherichia coli detection included in GB 5750-2006. It is currently the most advanced method for coliform detection in water, widely accepted by domestic testing authorities due to its convenience, speed, low false positive rate and suitability for high‑throughput rapid detection.
Compared with multiple-tube fermentation and membrane filtration, the enzyme substrate method greatly simplifies procedures, has low laboratory environment requirements, and shortens detection time to 24 hours. It is highly suitable for routine and emergency monitoring, enabling timely detection and risk prevention.
Table 1 Comparison of Multiple-tube Fermentation, Membrane Filtration and Enzyme Substrate Methods
表格
Item / MethodMultiple-tube FermentationMembrane FiltrationEnzyme Substrate Method
Detection Time3–5 days2–3 days1 day
False Positive—23–26%0.01%
Detection Range2–1600 MPN/100mL—1–2419.6 MPN/100mL
EnvironmentClean laboratory requiredClean laboratory requiredNo special requirements
Operator SkillProfessional & skilledProfessional & skilledSimple training sufficient
QuantitationMPN tableDirect countMPN table
Method15 tubesColony count51/97-well plate

V. Method Advantages

  1. No clean room required.

  2. Manual operation time < 1 minute.

  3. No medium preparation or extensive glassware sterilization needed.

  4. Qualitative and quantitative analysis completed within 24 hours; no confirmation test required.

The machine is used with MMO‑MUG medium (fixed substrate enzyme substrate method) specified in GB 5750‑2006, for sealing water samples in 51‑well or 97‑well quantitative trays.
Working Principle
A water sample containing target bacteria is added to Minimal Medium ONPG‑MUG and incubated at 36℃±1℃.

  • β‑galactosidase from total coliforms hydrolyzes ONPG, turning the medium yellow.

  • β‑glucuronidase from Escherichia coli hydrolyzes MUG, producing characteristic fluorescence.

  • Thermotolerant (fecal) coliforms hydrolyze ONPG at 44.5℃, turning the medium yellow.

VI. Product Features

  • High Efficiency & Energy Saving: Optimized heating element layout and temperature control algorithm improve efficiency and reduce power consumption.

  • Easy Operation: Intuitive touchscreen interface for parameter setting; self‑diagnosis for convenient maintenance.

  • Safe & Reliable: Overload protection, short‑circuit protection and multiple safety functions.

VII. Technical Specifications

  • Purpose: Detection of total coliforms, Escherichia coli, fecal coliforms in water by enzyme substrate method complying with GB 5750‑2006

  • Reliability: No leakage, no hole breakage

  • Stability: Supports > 50,000 samples; service life > 5 years

  • Convenience: Backspace key, quantitative tray counter, auto power saving, one‑key drain, large LCD, protective window, error alarm

  • Speed: No clean room needed; results in 24 h

  • Weight: 14.2 kg

  • Dimensions: 42 cm (L) × 41 cm (W) × 33 cm (H)

  • Preheating time: ≤ 3 min

  • Noise: < 48 dBA

  • Housing temperature: < 40℃

  • Sealing speed: 10 seconds

  • Power supply: AC 220 V ±10%, 50 Hz

  • Efficiency: ≤ 12 sec per tray (51‑well / 97‑well)

  • Operating temperature: -10℃ ~ 50℃

  • Detection range:

    • 51‑well tray: 0–200 MPN/100 mL (undiluted)

    • 97‑well tray: 0–2419 MPN/100 mL (undiluted)

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